Optimization of aqueous enzyme?assisted extraction of rosmarinic acid from rosemary ( <i>Rosmarinus</i> <i>officinalis</i> L.) leaves and the antioxidant activity of the extract

Rosemary (Rosmarinus officinalis L.) is a potential source of rosmarinic acid (RA). This study aimed to develop novel aqueous enzymatic approach for extracting RA from rosemary leaves and evaluate the antioxidant activity RA-rich extract. The ability several enzymes release plant tissues was investigated. Among examined enzymes, cellulase A exhibited highest extraction efficiency. Subsequently, A-assisted optimized. optimal conditions were as follows: an time 4.63 hr, water-to-sample ratio 28.69 ml/g, enzyme concentration 2.56%, temperature 36.6°C, with maximum content 13.97 mg/g. extract potent against 2,2-diphenyl-1-picrylhydrazyl radical IC50 value 532.01 ?g/ml. Aqueous cellulase-assisted green leaves. Furthermore, leaf promising agent future use. Practical applications widely applied in food pharmaceutical industries. developed new method involving use ecofriendly results indicated that nutraceutical practical process